Human Ceruloplasmin ELISA Kit - This kit is for the quantitative determination of total ceruloplasmin antigen in human plasma, serum, urine, milk, saliva and cell culture samples. For research use only. Strip well format for up to 96 tests. Human ceruloplasmin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti human ceruloplasmin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ceruloplasmin. Color development is proportional to the concentration of total ceruloplasmin in the samples. More Details Detection Target Ceruloplasmin Reactivity Human Range 1-1,000 ng/ml range Sensitivity 0.236 ng/mL